C₂₀H₁₄O₄
M.W: 318,32  CAS: 77-09-8  EC: 201-004-7  TARIC: 29322010
EC INDEX: 604-076-00-1  

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• Appearance A white / almost white powder
• Assay 98.0 – 101.0%
• Identification Conforms
• Loss on drying ? 0.5%
• Residue on roasting (SO₄) ? 0.1%
• Acidity or alkalinity Conforms
• Chloride (Cl^–) ? 0.01%
• Appearance of solution Conforms
• Sulfates (SO₄) ? 0.02%
• Transition range pH 8.2 – 10
• colourless to red-violet
• Practically insoluble in water

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C₁₆H₁₈ClN₃S·H₂O
C.I. Basic Blue 9, Tetramethylthionine Chloride
M.W: 319.85  CAS: 122965-43-9  EC: 200-515-2  TARIC: 32041300

H: H302  

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• Appearance ? Dark blue, crystalline powder with a copper-coloured sheen, or green crystals with a bronze-coloured sheen
• Assay 95.0 – 101.0%
• Identity ? passes test
• Solubility ? passes test
• Methanol insoluble subtances ? 1.0%
• Related substances ? passes test
• Iron (Fe) ? 0.02%
• Lead (Pb) ? 0.001%
• Zinc (Zn) ? 0.01%
• Sulphated ash (at 600ºC) ? 1.0%
• Loss on drying (105 °C) 8.0 – 22.0%

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C₁₆H₈N₂Na₂O₈S₂
Acid Blue 74 , Indigotine
M.W: 466,36   CAS: 860-22-0  EC: 212-728-8  TARIC: 32041200

H: H317  P: P261;P272;P280;P302+P352;P333+P313;P501  

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• Appearance ? Dark blue-violet powder having a metallic luster of copper
• Absorption maximum in water (H₂O) 608 – 612nm
• Absorptivity (A₁%; 1cm; ?_max; 0.001% H₂O) 380 – 475nm
• Loss on drying (110 °C) ? 10%
• Suitability for microbiology

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C₂₇H₂₈Br₂O₅S
BTB
M.W: 624.4  CAS: 76-59-5  EC: 200-971-2  TARIC: 29349990

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• Appearance Pink to purple coloured powder
• Appearance of solution Passes test
• pH Transition (6.0 – 7.6) Yellow to Blue
• Transition range (ACS) Passes test
• Sensitivity Passes test
• Loss on drying (110 °C) ? 3%

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