For one-step reverse transcription and PCR applications with gene-specific primers (GSP) for RNA targets. It is formulated with optimized components and buffers to enable reverse transcription and amplification in a single reaction tube. Supplied at a 2X concentration, it’s easy to use, requiring only the addition of GSP and RNA template, without the need to open caps or perform additional pipetting operations, saving time and effectively reducing the risk of contaminationÂ
PCR supplement that enhances sensitivity, leading to enhanced PCR effectiveness and decreased background noise. Especially beneficial for challenging templates (e.g., those rich in GC content) and intricate reactions
Optimized ready-to-use solution containing x-VITA Taq Polymerase for long fragments (TAQP-LT1-001), dNTPs, MgCl2 and stabilizers. It is inactive at Room Temperature and includes all material needed except Template and Primers
A specially formulated solution designed to amplify long DNA fragments. It combines two thermostable DNA polymerases, the standard Taq x-VITA and the proofreading x-VITA. It can amplify long DNA chains, such as the λ phage genome, of up to 20 kb. It’s also a superior choice for amplifying complex templates, particularly those with a high GC content. The elongation rate is 3 kb/min. The generated products consist of a mixture of 3′-dA overhang and blunt-end products, which can be used in TA cloning
Optimized ready-to-use solution containing x-VITA Taq Polymerase proofreading (TAQP-PF1-001), dNTPs, MgCl2 and stabilizers. It is inactive at Room Temperature and includes all material needed except Template and Primers
Derived from the hyperthermophilic archaeon Pyrococcus furiosus, it possesses superior thermostability and error correction properties (3′ to 5′ exonuclease activity), allowing it to correct nucleotide misincorporation errors. PCR products result in blunt ends, which are ideal for cloning into vectors. Elongation rate of 1 kb/min (70 – 75 °C)Â
The Hind III digest of lambda DNA yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis. ? DNA /Hind III is pre-mixed with loading buffer and is ready to useÂ
Pre-mixed loading buffer with tracking dyes for non-denaturing agarose and polyacrylamide gel electrophoresis. Dyes used include xylene cyanol, orange G, and bromophenol blue. Comes in 5 tubes, each containing 1 mlÂ
Proteinase K isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. It is a serine protease that exhibits broad cleavage specificity. With a molecular weight of 28.900 kDa, it cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. It is not inactivated by chelating reagents such as EDTA or detergents such as SDS and is active in a wide range of pH (4 – 12,5)
The Kit is designed to collect, and transport saliva specimens containing viruses from patients with signs and symptoms of respiratory infection and subsequent sample processing prior Direct PCR. Saliva sample are collected by spitting inside the collection funnel which has been assembled with the collection tube. Researchers at Yale University developed a saliva-based method for the detection of SARS-CoV-2. The method uses proteinase K, followed by a heat step to make viral RNA detectable in a saliva sample instead of using kits to extract the RNA. It includes the PK solution (containing proteinase K) that has been specifically formulated to be used following the open-source protocol developed by YaleÂ’s researchers and obtain PCR-ready nucleic acids from saliva
Designed for the rapid purification of viral RNA from cell-free samples such as serum, plasma, urine, cell free body fluids, cell culture supernatants and rinse liquid from swabs samples. Viral RNA molecules bind to the silica-based media and impurities such as proteins and nucleases are removed by thorough washing with Wash Buffer. The RNA is then eluted in sterile, RNase free water. The isolated Viral RNA is ready-to-use and should be stored at -70°C. The procedure can be used for isolation of viral RNA from a broad range of viruses. However, performance cannot be guaranteed for every virus species and must be validated by the customer. The amount of purified viral RNA depends on the sample type, the virus titer, sample source, transport, storage, and age. The Kit also includes carrier RNA that improves binding and recovery of low-concentrated viral RNA
Reliable, easy-to-use and rapid method for high-quality genomic DNA purification from various sources, including: whole blood, buffy coat or cultured cells. The procedure includes: lysis, protein removal, DNA precipitation, washing and hydration
This purification kit provides an accurate, easy-to use and rapid method to isolate high quality DNA from both Gram negative and Gram positive bacteria. The kit uses breakthrough technology based in the ability to bind silica in the presence of high concentrations of chaotropic salts as guanidinium thiocyanate. The extraction process uses comfortable MiniSpin Columns and includes an initial cell-wall lysis step with the appropriate enzyme to ensure efficient cell lysis and DNA release from the cell
Reliable, easy and rapid Mini Spin Kit for the purification of high-quality DNA from human and animal sample saliva. The kit is based in the DNA ability to bind silica in the presence of high concentrations of chaotropic salts
