Dyes and reagents – Isometric

Dyes and reagents

Dyes and stains kits for microbiology and pathology

Ready-to-use solutions of some of the most widely used stains in the fields of histopathology, microbiology and hematology. Labkem dye kits are fast and easy to use, ensuring reproducible results. Reagents in 50 ml format

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Hematoxylin 80% (C.I. 75290) MIC
C₁₆H₁₄O₆
M.W: 302,30 CAS: 517-28-2 EC: 208-237-3 TARIC: 32030010

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Appearance Beige to tan to light brown powder

Assay ? 60%

Water (H₂O) by Karl – Fischer ? 8%

Suitable as a Biological Stain passes test

General tissue stain for animal histology by Weigerts Mayers acid hemalum Heidenhain’s and Harris technique on paraffin sections.

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Fuchsin basic (C.I. 42510) MIC
C₂₀H₂₀ClN₃
Basic Violet 14
M.W: 337,85 CAS: 632-99-5 EC: 211-189-6 TARIC: 32041300

H: H351

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Appearance Dark-Green Crystalline Powder

Loss on drying (110 °C) ? 10%

Dye content ? 80%

Spectroscopy (50% EtOH) 549nm

Melting point ? 250ºC

Transition range pH 1.0 – 3.1

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Eosin aqueous solution 1%, MIC
CAS: 548-26-5 TARIC: 32041300

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Appearance Liquid

Colour Orange

Odour Odourless

Specific gravity 1.0

Solubility Soluble in water

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Wright
Eosin Methylene blue according to Wright
CAS: 68988-92-1 EC: 273-541-5 TARIC: 32041900

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Appearance ? Green Powder

Loss on drying (105 °C) ? 10%

Solubility 0.1 % (MeOH) ? Clear blue solution

Absorbance maxima at 1( methanol) 648 – 653nm

Absorbance maxima at 2( methanol) 520 – 525nm

Specific absorptivity 1%/1cm at 1 (dried subs.) 1100 – 1300

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Toluidine blue (C.I. 52040) MIC
C₁₅H₁₆ClN₃S
M.W: 305,82 CAS: 92-31-9 EC: 202-146-2 TARIC: 32041300

P: P102-P202

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Appearance Dark-green to black powder

Assay ? 80%

Absorption maximum in water (H₂O) ? 1200nm

Loss on drying (110 °C) ? 8%

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Orange G (C.I. 16230) MIC
C₁₆H₁₀N₂Na₂O₇S₂
Acid Orange 10
M.W: 452,37 CAS: 1936-15-8 EC: 217-705-6 TARIC: 32041200

P: P102+P202

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Appearance Red-orange powder

Absorption maximum in water (H₂O) 475 – 481nm

Dye content ? 80%

Soluble in water and methyl Cellosolve, slightly soluble in ethanol.

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Lactophenol (cotton blue) solution MIC
TARIC: 38229000

UN: 2922 IMDG: 6.1 (8) II UN 2927 ADR: 6.1 (8) II UN 2927 (D/E) IATA: 6.1 (8) II UN 2927

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Suitability for microbiology Passes test

Solubility Passes test

Blue solution for staining fungi

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Giemsa
CAS: 51811-82-6 EC: 257-438-2 TARIC: 32041900

H: H319

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Appearance Dark green to black powder

Abs. (524nm) ? 600

Abs. (651 nm) ? 950

Loss on drying (110 °C) ? 10%

Melting point ? 300ºC

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Fuchsin acid (C.I. 42685) MIC
C₂₀H₁₇N₃Na₂O₉S₃
Ac. Violet 19, Fuchsin S, Rubin Acid, Rubin S
M.W: 585.54 CAS: 3244-88-0 EC: 221-816-5 TARIC: 32041200

P: P102-P202

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Appearance/color ? Dark green powder/ Olive, green crystals with bronze metallic lustre

Absortion 540 – 543nm

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Formaldehyde 3.7-4.0% prefilled container HIS buffered pH=7
CH₂O
Neutral Buffered Formaline, NBF
M.W: 30.03 CAS: 50-00-0 EC: 200-001-8 EC INDEX: 603-001-00-X

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Assay 3.7 – 4%

Content of MeOH 1 – 1.5% w/v

pH 6.8 – 7.2

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Fluorescein sodium (C.I. 45350) MIC, BP
C₂₀H₁₀Na₂O₅
Acid Yellow 73, Uranina
M.W: 376,30 CAS: 518-47-8 EC: 208-253-1
P: P102+P202

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Appearance/color A dark red crystalline powder

Assay ? 98%

Lead (Pb) ? 0.002%

Cadmium (Cd) ? 0.001%

Copper (Cu) ? 0.01%

Zinc (Zn) ? 0.01%

Arsenic (As) ? 0.0002%

Acriflavine (C₁₄H₁₄ClN₃) Passes test

Loss on drying ? 7%

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Alcian Blue 8GX (C.I. 74240) MIC
C₅₆H₆₈Cl₄CuN₁₆S₄
Ingrain Blue 1
M.W: 1298.86 CAS: 33864-99-2 EC: 251-705-7 TARIC: 32041700

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Appearance Dark Blue Powder

Absorption maximum 600 – 615nm

Dye content ? 50%

Water (H₂O) by Karl – Fischer ? 35%

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Aniline blue (C.I. 42755) water soluble MIC
C₃₂H₂₅N₃Na₂O₉S₃
Acid blue 22, China blue, Methyl blue
M.W: 737.72 CAS: 28631-66-5 EC: 249-113-9 TARIC: 32041200

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Appearance Brownish-purple crystalline powder With metallic lustre

Absorption Maxima in (0.005 mol/L HCl) 594 – 610nm

Solubility in water (H₂O)

Used with fuchsin acid as Mallory’s connective tissue stain visualizes chromosomes and cellulose wal

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