Labbox – Page 171 – Isometric

Labbox

Agarose MS-4 GEN
CAS: 9012-36-6 EC: 232-658-1 TARIC: 13023100

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Appearance White cream powder

Transparence (3%) ? 6NTU

pH in solution 6.0 – 8.0

pH in gel 6.0 – 8.0

Residue on calcination ? 0.3%

Sulfates (SO₄) ? 0.11%

Gel strength (3%) ? 500g/cm

Melting point (3%) ? 76°C

Gel point (3%) ? 31°C

Gel strength (5%) ? 1000g/cm

Particle size (mesh 60 ASTM) 0.3 – 1.0%

Particle size (mesh 100 ASTM) 5.0 – 10.0%

EEO (Electroendosmosis) ? 0.12

Applications:

Fine resolution, Protein electrophoresis.

Storage temperature 2 – 25°C

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Agarose LM-SIEVE GEN
CAS: 9012-36-6 EC: 232-658-1 TARIC: 13023100

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Residue on calcination ? 0.3%

DNase/RNase activity Non-detected

EEO (Electroendosmosis) ? 0.1%

Assay moisture ? 10%

pH in gel ? 9

pH in solution ? 9

Colour White

Appearance Fine, homogeneus powder

Particle size (A.S.T.M) over sieve 60 ? 2%

Particle size (A.S.T.M) over sieve 200 ? 28%

Particle size (A.S.T.M) under sieve 200 ? 14%

DNA binding None detected

Comparative assay of different size DNA fragments Bands appear sharp and finely resolved

Background fluorescence assay in ethidium bromide Very low gel background after EtBr staining

In-Gel restriction and ligation (assayed enzymes: EcoRI, HindIII and DNA t4 ligase) Passes test

Digestion with agarase enzyme and DNA recovery Passes test

Gel strength 4% ? 1000g/cm2

Gel point (4%) ? 35ºC

Melting point (4%) ? 65ºC

Clarity of solution ? 9NTU

Sulfate ? 0.12%

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Agarose E GEN
CAS: 9012-36-6 EC: 232-658-1 TARIC: 13023100

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Appearance White cream powder

Loss on drying ? 7%

pH in solution 6.75 – 6.95

pH in gel 6.75 – 6.95

Residue on calcination ? 0.45%

colourimetry (1.5%, 450 nm) ? 0.032

Gel strength 1% ? 1000g/cm²

Turbidity (1.5%) ? 4NTU

Sulfates (SO₄) ? 0.15%

Gel strength 1.5% ? 2000g/cm²

Particle size (mesh 100 ASTM) 28.5%

Gel point (1.5%) 34.5 – 37.5°C

Particle size (mesh 60 ASTM) 0.65%

Melting range (1.5%) 86.5 – 89.5°C

Analytical separation ? 1000bp

EEO (Electroendosmosis) 0.05 – 0.13

Applications:

Blotting

Separations of nucleic acid fractions from 250bp to 23kb

Storage temperature 2 °C – 25 °C

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Agar agar BAC
CAS: 9002-18-0 EC: 232-658-1 TARIC: 13023100

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Appearance White to pale brown powder

Particle size (mesh 60 ASTM) ? 95%

Loss on drying ? 12%

Residue on calcination ? 5%

Turbidity 1.5 % Before autoclaving ? 8NTU

pH (1.5%) before autoclaving 6.0 – 7.5

pH (1.5%) after autoclaving 6.0 – 7.5

Colourimetry before autoclaving (450 nm) ? 0.25

Colourimetry after autoclaving (450 nm) ? 0.30

Gel strenght (Nikan method) Before autoclaving 800 – 1100g/cm²

Gel point (1.5%) 34 – 38°C

Melting range (1.5%) 85 – 90°C

Concentration of use 1.2 % – 1.6 %

Homogeneous, free flowing and beige. If any changes phisically, discard product.

Absernce of inhibitors

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Zinc sheet 85 x 25 x 1 mm GLR
Zn
M.W: 65.38 CAS: 7440-66-6 EC: 231-175-3 EC INDEX: 030-001-00-1

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Approximated dimensions 100 x 30 x 0.8 mm

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Zinc, powder AGR
Zn
M.W: 65.38  CAS: 7440-66-6  EC: 231-175-3  TARIC: 79039000
EC INDEX: 030-001-00-1
UN: 3077  IMDG: 9 III UN 3077  ADR: 9 III UN 3077   IATA: 9 III UN 3077  H: H410  P: P273

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Assay ? 99%

Arsenic (As) ? 0.00002%

Iron (Fe) ? 0.05%

Lead (Pb) ? 0.05%

Tin (Sn) ? 0.01%

Chloride (Cl^–) ? 0.005%

Cadmium (Cd) ? 0.005%

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Zinc nitrate hexahydrate AGR ACS
Zn(NO₃)₂·6H₂O
M.W: 297,47 CAS: 10196-18-6 EC: 231-943-8 TARIC: 28342980

UN: 1514 IMDG: 5.1 II UN 1514 ADR: 5.1 II UN 1514 (E) IATA: 5.1 II UN 1514

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Assay ? 98%

Substances insoluble in water (H2O) ? 0.005%

Iron (Fe) ? 0.0005%

Chloride (Cl^–) ? 0.001%

Nickel (Ni) ? 0.0005%

Copper (Cu) ? 0.0005%

Calcium (Ca) ? 0.001%

Sulfates (SO₄) ? 0.005%

Magnesium (Mg) ? 0.001%

Potassium (K) ? 0.001%

Sodium (Na) ? 0.001%

Lead (Pb) ? 0.001%

Cadmium (Cd) ? 0.0005%

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Zinc nitrate hexahydrate EPR
Zn(NO₃)₂·6H₂O
M.W: 297,47 CAS: 10196-18-6 EC: 231-943-8 TARIC: 28342980

UN: 1514 IMDG: 5.1 II UN 1514 ADR: 5.1 II UN 1514 (E) IATA: 5.1 II UN 1514

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Assay (Zn) ? 98.0%

Appearance White crystals

Substances insoluble in water (H2O) ? 0.01%

Iron (Fe) ? 0.005%

Chloride (Cl^–) ? 0.005%

Free acid (HNO₃) ? 0.04%

Calcium (Ca) ? 0.02%

Sulfates (SO₄) ? 0.01%

Potassium (K) ? 0.02%

Sodium (Na) ? 0.02%

Lead (Pb) ? 0.02%

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Zinc chloride, anhydrous AGR
ZnCl₂
M.W: 136,30  CAS: 7646-85-7  EC: 231-592-0  TARIC: 28273985
EC INDEX: 030-003-00-2
UN: 2331  IMDG: 8 III UN 2331  ADR: 8 III UN 2331 (E)  IATA: 8 III UN 2331

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Assay ? 98%

Oxychlorides (ZnO) ? 1.2%

Iron (Fe) ? 0.0005%

Total Nitrogen ? 0.002%

Calcium (Ca) ? 0.001%

Copper (Cu) ? 0.001%

Sulfates (SO₄) ? 0.002%

Magnesium (Mg) ? 0.001%

Potassium (K) ? 0.001%

Sodium (Na) ? 0.001%

Lead (Pb) ? 0.001%

Cadmium (Cd) ? 0.0005%

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Zinc chloride, anhydrous EPR
ZnCl₂
M.W: 136,30 CAS: 7646-85-7 EC: 231-592-0 EC INDEX: 030-003-00-2
UN: 2331 IMDG: 8 III UN 2331 ADR: 8 III UN 2331 (E) IATA: 8 III UN 2331

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Assay ? 98%

Oxychlorides (ZnO) ? 2.4%

Iron (Fe) ? 0.002%

Sodium (Na) + Potassium (K) + Calcium (Ca) ? 1%

Copper (Cu) ? 0.002%

Lead (Pb) ? 0.002%

Cadmium (Cd) ? 0.001%

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Wright
Eosin Methylene blue according to Wright
CAS: 68988-92-1 EC: 273-541-5 TARIC: 32041900

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Appearance ? Green Powder

Loss on drying (105 °C) ? 10%

Solubility 0.1 % (MeOH) ? Clear blue solution

Absorbance maxima at 1( methanol) 648 – 653nm

Absorbance maxima at 2( methanol) 520 – 525nm

Specific absorptivity 1%/1cm at 1 (dried subs.) 1100 – 1300

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Water LC/MS LGR
H₂O
M.W: 18.02 CAS: 7732-18-5 EC: 231-791-2 TARIC: 28539010

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LC-MS Suitability

ESI+ mode (as Reserpine) ? 50ppb

ESI- mode (as 4-Nitrophenol) ? 50ppb

Residue after evaporation ? 1ppm

UV Absorbance at 200 nm ? 0.01

UV Absorbance at 250-400 nm ? 0.005

Gradient Elution test

Largest eluted peak (254 nm) ? 1mAU

Largest eluted peak (210 nm) ? 5mAU

Fluroescence Trace Impurities

-at 450 nm Emission ? 0.1ppb

-at Emission Maximum for impurities ? 0.2ppb

Aluminium (Al) ? 25ppb

Barium (Ba) ? 5ppb

Cadmium (Cd) ? 5ppb

Calcium (Ca) ? 25ppb

Chromium (Cr) ? 5ppb

Cobalt (Co) ? 5ppb

Copper (Cu) ? 5ppb

Iron (Fe) ? 5ppb

Lead (Pb) ? 5ppb

Magnesium (Mg) ? 5ppb

Manganese (Mn) ? 5ppb

Lithium (Li) ? 5ppb

Nickel (Ni) ? 5ppb

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Water ultrapure AGR
H₂O
M.W: 18.02 CAS: 7732-18-5 EC: 231-791-2 TARIC: 28539010

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H2O-LF ? 1µS/cm*

*At the bottling time

Iron (Fe) ? 0.0000001%

UV filtrated 0.4µm

Non-volatile matter ? 0.0001%

Chloride (Cl^–) ? 0.000005%

Copper (Cu) ? 0.00000004%

Aluminum (Al) ? 0.0000002%

Fluorhide (F) ? 0.000005%

Sulfates (SO₄) ? 0.00001%

Nitrates (NO₃) ? 0.000001%

Potassium (K) ? 0.0000005%

Phosphate (PO₄) ? 0.000001%

Silica (SiO₂) ? 0.000001%

Silver (Ag) ? 0.00000004%

Cadmium (Cd) ? 0.0000001%

Arsenic (As) ? 0.0000002%

Gold (Au) ? 0.0000001%

Boron (B) ? 0.0000005%

Barium (Ba) ? 0.0000001%

Beryllium (Be) ? 0.0000002%

Bismuth (Bi) ? 0.0000001%

Calcium (Ca) ? 0.0000005%

Cobalt (Co) ? 0.0000001%

Chromium (Cr) ? 0.00000004%

Lithium (Li) ? 0.0000002%

Magnesium (Mg) ? 0.0000005%

Manganese (Mn) ? 0.00000004%

Molybdenum (Mo) ? 0.0000002%

Sodium (Na) ? 0.000001%

Nickel (Ni) ? 0.00000004%

Lead (Pb) ? 0.0000001%

Platinum (Pt) ? 0.0000001%

Antimony (Sb) ? 0.0000001%

Selenium (Se) ? 0.00000001%

Tin (Sn) ? 0.0000001%

Strontium (Sr) ? 0.00000004%

Titanium (Ti) ? 0.0000001%

Vanadium (V) ? 0.0000001%

Zinc (Zn) ? 0.0000004%

Zirconium (Zr) ? 0.0000001%

pH 5.0 – 7.5

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Water EPR Ph.Eur.
H₂O
M.W: 18.02 CAS: 7732-18-5 EC: 231-791-2

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pH 5 – 6.5

Conductivity at 20 °C ? 3.5µS/cm*

Microbial contamination (TAMC) ? 100CFU/ml

Heavy metals (Pb) ? 0.00001%

Nitrates (NO₃) ? 0.00002%

Oxidizable substances Passes test

Sulfates (SO₄) Passes test

Total Organic Carbon (TOC) ? 0.00001%

Acidity or alkalinity Passes test

Chloride (Cl^–) Passes test

Ammonium (NH₄) ? 0.00002%

Calcium (Ca) + Magnesium (Mg) Passes test

Residue on evaporation ? 0.001%

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Vanillin 99.9% AGR
C₈H₈O₃
M.W: 152,15 CAS: 121-33-5 EC: 204-465-2 TARIC: 29124100

H: H319

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Assay ? 99.9%

Melting range 81 – 83°C

Substances insoluble in Ethanol (CH3CH2OH) ? 0.01%

Residue on roasting (SO₄) ? 0.01%

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